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# Welcome to DICE

## DICE

DICE (Dimensional Inferences from Coarse-grained Ensembles)

The DICE web tool computes the radius of gyration, $$R_g$$, of disordered or denatured peptide chains based on single-molecule FRET data and an unbiased homopolymer model. In the direct mode, the output is either the most likely $$R_g$$ value ($$R_g^0$$) or a distribution of $$R_g$$ values that is consistent with the experimental FRET efficiency (E), which can be input either as a single value (mean) or as a distribution of E. In the reverse mode, the program estimates the expected FRET efficiency and the distribution of $$R_g$$ based on a distribution of end-to-end distances; for example derived from coarse-grained simulations, an analytical expression, or from Protein Ensemble Database (pE-DB) conformations. For more details, see Song, Gomes, Shi, Gradinaru & Chan, Biophys. J. (2017), and Song, Gomes, Gradinaru & Chan, J. Phys. Chem. B (2015).